Abstract

CABRERA MORALES, C. M.. Comparative studies of HER2/neuamplification by means of FISH in real-time quantitative PCR in breast cancer. Oncología (Barc.) [online]. 2005, vol.28, n.10, pp.26-30. ISSN 0378-4835.

The HER2/neu protooncogene is amplified or over-expressed in 20 to 30% of breast tumors and its determination is important to evaluate the susceptible patients to be treated with trastuzumab. We compare in this study the FISH and real-time quantitative PCR techniques in the determination of HER2/neu amplification of 40 breast tumors. The FISH procedure showed 21 FISH-positive and 19 FISH-negative tumors. These results proved to have a good concordance with the PCR technique, that was of 100% for the FISH-negative and 80.95% for the FISH-positive breast tumors. Therefore, the real-time quantitative PCR represents a useful technique for establishing the HER2/neu status in breast cancer.

Keywords : Breast cancer; HER2/neu; FISH; Real.time quantitative PCR.

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