Abstract

SAPIDIS, Nikolaos et al. The effect of glutamine and synbiotics on the healing of colonic anastomosis. Rev. esp. enferm. dig. [online]. 2014, vol.106, n.4, pp.255-262. ISSN 1130-0108.

Introduction: Intestinal wound healing is an essential process for surgical reconstruction of the digestive tract. The purpose of this study is to evaluate the effect of perioperative administration of glutamine and synbiotics on the biological behavior of intestinal mucosal barrier and the healing of colonic anastomosis in rats. Material and methods: 80 Wistar rats were divided in five groups. A: Control. B: Mechanical bowel preparation and antibiotics. C: Glutamine. D: Synbiotics. E: Glutamine and synbiotics. The animals were sacrificed on 3^rd and 7^th postoperative day. Results: Zero mortality and no septic complications were noted. On 3^rd postoperative days, a significant weight loss was observed in all groups in comparison with the preoperative weights, but on the 7^th day in groups C and E, in contrast with the other groups, weight loss was not significant. On the 3^rd postoperative day, neoangiogenesis, inflammatory infiltration and fibroblast activity were significantly enhanced in group E compared to control. On the 7^th postoperative day in group E fibroblast activity was significantly enhanced and inflammatory infiltration was significantly limited compared to control. The bursting pressures as well as the hydroxyproline tissue content were significantly higher in the group E on 3^rd and 7^th postoperative days. The percentage of positive mesenteric lymph node cultures were significantly limited in group E compared to control. Conclusions: The administration of synbiotics in conjunction with glutamine resulted in increasing the mechanical strength of the anastomosis, thus increasing the bursting pressure and decreasing or effacing of anastomotic dehiscence and limiting bacterial translocation.

Keywords : Adhesion; Bursting pressure; Colon; Hydroxyproline.

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