Abstract

BLANCO PAMPIN, J.; GARCIA RIVERO, S.A.  and  HINOJAL FONSECA, R.. Structural proteins and mediators of inflammation: markers for the diagnostic postmortem of myocardial ischemia (inmunohistochemical study). Cuad. med. forense [online]. 2004, n.35, pp.51-70. ISSN 1988-611X.

Postmortem histopathological diagnosis of myocardial infarction has many problems in human material coming from autopsy. Until the present moment, this matter hasn’t been resolved by conventional histological procedures (hematoxilin-eosin, Masson´s trichrome and histochemical techniques). Another disadvantage of the enzyme-histohemical technique is that it is only applicable to un-fixed specimens. In this study, 50 myocardial tissue specimens were taken at autopsy from victims who died of sudden cardiac death. In addition, six cases of macroscopic myocardial infarction (positive controls) and 8 cases of rapid and non-cardiac causes of death (negative controls) were included in the study. The expression of actin, desmin, myoglobin and Complement factor (C5b-9) by avidin-biotin-peroxidase complex method, and its possible utility in post-mortem diagnosis of human heart failure, were studied. Results of our study, show that the inmunohistochemical method using formalin-fixed, paraffin-embebed tissue is valuable for post-mortem detection of myocardial ischemia.

Keywords : Sudden cardiac death; post-mortem diagnosis; inmunohistochemistry; myocardial ischemia; medicolegal autopsy.

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