Abstract

MARTIN-LOPEZ, Juana V. et al. Simultaneous PCR detection of ica cluster and methicillin and mupirocin resistance genes in catheter-isolated Staphylococcus. INT. MICROBIOL. [online]. 2004, vol.7, n.1, pp.63-66. ISSN 1139-6709.

Recent data show that more than 50% of catheter-associated bloodstream infections are caused by staphylococci. Staphylococcal infections produced by intercellular-adhesion cluster (ica) carriers can be even more problematic due to the presence of methicillin and mupirocin resistance genes. In the present study, a multiplex PCR protocol that allows the simultaneous identification of staphylococci and detection of both the ica and methicillin and/or mupirocin resistance genes was developed. Furthermore, the method allows differential detection of the ica locus from Staphylococcus aureus and Staphylococcus epidermidis.

Keywords : Staphylococcus aureus; Staphylococcus epidermidis; intercellular adhesion gene cluster; antibiotic resistance; multiplex PCR.

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