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Sanidad Militar

Print version ISSN 1887-8571


SANMARTIN SANCHEZ, L.; LOZANO BENITO, D.; RICO SEVILLA, M.  and  VEGA-PLA, J.L.. Hair cortisol detection as biomarker of chronic stress in military working dogs. Sanid. Mil. [online]. 2016, vol.72, n.4, pp.255-259. ISSN 1887-8571.

Justification: The measurement of hair cortisol, is a noninvasive technique that provides a retrospective of its accumulation over a period of time. Objective: To investigate the levels of cortisol in dogs as a biomarker of chronic stress, and the effect of factors such as season and deployment. Design: The study was conducted in the LIA, with a total of 24 dogs subjected to differential environmental conditions. Methods: Hair samples were analyzed by ELISA for measuring cortisol concentration after alcoholic extraction. Results: The mean concentration of cortisol in dogs was significantly higher in spring than summer, and no significant effects of deployment were found. Conclusion: This study reveals different levels of cortisol concentration in hair dog´s between seasons.

Keywords : Dog; Hair Cortisol; Stress; Season; Deployment; ELISA.

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