SciELO - Scientific Electronic Library Online

vol.19 issue1Nutritional status in Mexican elderlys: comparative study between groups with different social assistances author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand




Related links

  • On index processCited by Google
  • Have no similar articlesSimilars in SciELO
  • On index processSimilars in Google


Revista Española de Nutrición Humana y Dietética

On-line version ISSN 2174-5145Print version ISSN 2173-1292


MENAL-PUEY, Susana  and  ASENSIO, Esther. Validation of a rapid method for detecting nitrate in chard (Beta vulgaris cycla): analysis of Spanish commercial samples marketed in the Region of Huesca, Spain, and estimation of the daily intake. Rev Esp Nutr Hum Diet [online]. 2015, vol.19, n.1, pp.4-11. ISSN 2174-5145.

Introduction: This paper presents the validation of a rapid method for the determination of nitrate content in chard samples, in order to determine, during two periods (winter and summer of 2012), the current levels of nitrate in this typical vegetable, and the toxicological risk associated with this intake. Material and Methods: A rapid colorimetric determination of nitrate in chard samples by nitration of salicylic acid was validated. The validated method was applied to analyze the content of nitrate in 56 chard samples marketed in Huesca (Spain) and collected in winter and summer seasons, and the toxicological risk associated with the intake for adult and children population was evaluated. Results: The method was specific and robust enough for the required purposes. The main performance characteristics of the method were: limits of detection and quantitation of 0.29 mg L-1 and 0.59 mg L-1, respectively; recoveries from 80.0% to 107.4%; and coefficients of variation lower than 11.4%. The detected mean nitrate content was 2293 mg kg-1 and there was evidence of risk only for extreme consumers (adults and children), especially in winter period. Conclusions: A high percentage of chard samples with a considerable concentration of nitrate were found. Taking into account the estimated dairy intake of nitrate associated with them, it could be recommended to establish a regulatory limit of nitrate to chard, a vegetable of important consumption in Spain.

Keywords : Nitrate; Vegetables; Contamination; Dietary intake; Toxicological risk.

        · abstract in Spanish     · text in English     · English ( pdf )


Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License