My SciELO
Custom servicesServices on Demand
Journal
Article
English (pdf)
Article in xml format
Article references
Automatic translation
Send this article by e-mailIndicators
Cited by SciELO 
Access statistics
Related links
Cited by Google 
Similars in
SciELO 
Similars in Google
Share
Permalink
Ars Pharmaceutica (Internet)
On-line version ISSN 2340-9894
Abstract
RESTREPO-ESPINOSA, Diana C. et al. Anticoagulant properties of a high molecular weight polysaccharide fraction (1000RS) of the ascidian Microcosmus exasperatus. Ars Pharm [online]. 2017, vol.58, n.3, pp.95-101. Epub Dec 12, 2022. ISSN 2340-9894. https://dx.doi.org/10.30827/ars.v58i3.6526.
Aims:
The anticoagulant effect and cytotoxicity of a high molecular weight polysaccharide fraction (1000RS) obtained from the tunic of the ascidia Microcosmus exasperatus were evaluated.
Methods:
Anticoagulant properties of 1000RS was evaluated by activated Partial Thromboplastin Time (aPTT), Thrombin Time (TT), Prothrombin Time (PT), anti-factor Xa and lupic anticoagulant (dRVVT) assays. Cytotoxicity was tested on murine hematopoietic cells using MTT assay.
Results:
This galactose rich fraction showed to be a potential anticoagulant due to its inhibitory effect on the intrinsic coagulation pathway. At the same time, anticoagulant doses of this fraction have no effect on cellular viability, which means that it can be used as a therapeutic agent.
Conclusion:
In vitro anticoagulant effect of 1000RS occurs at innocuous doses, however, it still need to be tested using in vivo models and its cytotoxicity evaluated in normal human cell lines.
Keywords : anticoagulants; ascidian; Microcosmus exasperatus; polysaccharides.
