<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>1887-8571</journal-id>
<journal-title><![CDATA[Sanidad Militar]]></journal-title>
<abbrev-journal-title><![CDATA[Sanid. Mil.]]></abbrev-journal-title>
<issn>1887-8571</issn>
<publisher>
<publisher-name><![CDATA[Ministerio de Defensa]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S1887-85712017000300147</article-id>
<article-id pub-id-type="doi">10.4321/s1887-85712017000300002</article-id>
<title-group>
<article-title xml:lang="es"><![CDATA[Investigación de Escherichia Coli productor de toxinas Shiga (STEC) en carnes y derivados cárnicos]]></article-title>
<article-title xml:lang="en"><![CDATA[Researching Escherichia Coli as a Shiga (STEC) toxin producer in meat and meat products]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Rípodas Navarro]]></surname>
<given-names><![CDATA[A.]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Fernández Moreira]]></surname>
<given-names><![CDATA[D.]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Macho Martínez]]></surname>
<given-names><![CDATA[M.]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Centro Militar de Veterinaria de la Defensa Servicio Bromatología y Seguridad Alimentaria ]]></institution>
<addr-line><![CDATA[Madrid ]]></addr-line>
<country>España</country>
</aff>
<aff id="Af2">
<institution><![CDATA[,Centro Militar de Veterinaria de la Defensa Servicio Bromatología y Seguridad Alimentaria ]]></institution>
<addr-line><![CDATA[Madrid ]]></addr-line>
<country>España</country>
</aff>
<aff id="Af3">
<institution><![CDATA[,Delegación de Defensa de Madrid  ]]></institution>
<addr-line><![CDATA[Madrid ]]></addr-line>
<country>España</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>09</month>
<year>2017</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>09</month>
<year>2017</year>
</pub-date>
<volume>73</volume>
<numero>3</numero>
<fpage>147</fpage>
<lpage>152</lpage>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://scielo.isciii.es/scielo.php?script=sci_arttext&amp;pid=S1887-85712017000300147&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://scielo.isciii.es/scielo.php?script=sci_abstract&amp;pid=S1887-85712017000300147&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://scielo.isciii.es/scielo.php?script=sci_pdf&amp;pid=S1887-85712017000300147&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="es"><p><![CDATA[RESUMEN  Introducción: El término Escherichia coli productor de toxina Shiga (STEC) abarca un grupo de patógenos alimentarios emergentes, implicados en numerosos brotes a nivel mundial. Son responsables de infecciones y enfermedades gastrointestinales graves tales como el síndrome urémico hemolítico (SUH) y la colitis hemorrágica (CH). Muchos alimentos han sido vinculados a estos brotes, destacando las carnes y derivados cárnicos crudos o insuficientemente cocinados.  Objetivo: Evaluar la prevalencia de STEC en carnes y derivados, de especies de abasto y cinegéticas, consumidas dentro de las Fuerzas Armadas (FAS).  Material y Métodos: Se analizaron un total de 170 muestras recibidas en el Servicio de Bromatología y Seguridad Alimentaria, del Centro Militar de Veterinaria. Para determinar la presencia de STEC se empleó la técnica de PCR a tiempo real mediante el uso del kit comercial Custom TaqMan ISO STEC Screening Assay (Thermo Scientific&#8482;), siguiendo las instrucciones del fabricante. Para el análisis estadístico se utilizó el programa SPSS®.  Resultados: La prevalencia de STEC en el total de las muestras analizadas fue de un 19,41 %. La hallada entre los preparados cárnicos de especies de abasto fue de un 25,42 %, mientras que la de derivados de carne de caza, fue de un 27,4 %. Los resultados obtenidos del cribado de STEC mostraron significación estadística.  Conclusiones: Los resultados obtenidos concuerdan con la mayoría de estudios anteriormente publicados, recalcando la importancia que representa el control de este grupo de patógenos, pudiéndose ampliar el espectro de matrices a analizar, dada la potencial exposición de los consumidores de las FAS a estos agentes.]]></p></abstract>
<abstract abstract-type="short" xml:lang="en"><p><![CDATA[SUMMARY  Introduction: Shiga toxin-producing Escherichia coli (STEC) is made up of a group of emerging food pathogens, implicated in numerous outbreaks worldwide. They are responsible for infections and serious gastrointestinal diseases such as hemolytic uremic syndrome (HUS) and hemorrhagic colitis (HC). Many foods have been linked to these outbreaks, with emphasis on raw or undercooked meat and meat products.  Aim: To evaluate the prevalence of STEC in meat and meat products from food-producing and game species, consumed within the Armed Forces (AF).  Material and Methods: We analyzed a total of 170 samples received in the Service of Bromatology and Food Safety, of the Veterinary Military Center. To determine the presence of STEC, real-time PCR technique was performed using the &#8220;Custom TaqMan ISO STEC Screening Assay&#8221; kit (Thermo Scientific &#8482;), following the manufacturer&#8217;s instructions. Statistical analysis was carried out using the SPSS® program.  Results: The prevalence of STEC in the total amount of samples analyzed was 19.41%. Prevalence of meat products from food-producing species was 25.42%, whereas the one from game meat was 27.4%. The results obtained from the STEC screening showed statistical significance.  Conclusions: The results obtained agree with most of previously published studies, emphasizing the importance of surveillance. Expanding the spectrum of matrices to analyze could be an interest approach, given the potential exposure of AF consumers to these agents.]]></p></abstract>
<kwd-group>
<kwd lng="es"><![CDATA[Escherichia coli STEC]]></kwd>
<kwd lng="es"><![CDATA[PCR a tiempo real]]></kwd>
<kwd lng="es"><![CDATA[carnes y derivados cárnicos]]></kwd>
<kwd lng="es"><![CDATA[prevalencia]]></kwd>
<kwd lng="es"><![CDATA[Fuerzas Armadas]]></kwd>
<kwd lng="en"><![CDATA[Escherichia coli STEC]]></kwd>
<kwd lng="en"><![CDATA[real-time PCR]]></kwd>
<kwd lng="en"><![CDATA[meat and meat products]]></kwd>
<kwd lng="en"><![CDATA[prevalence]]></kwd>
<kwd lng="en"><![CDATA[AF]]></kwd>
</kwd-group>
</article-meta>
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