<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>2340-9894</journal-id>
<journal-title><![CDATA[Ars Pharmaceutica (Internet)]]></journal-title>
<abbrev-journal-title><![CDATA[Ars Pharm]]></abbrev-journal-title>
<issn>2340-9894</issn>
<publisher>
<publisher-name><![CDATA[Universidad de Granada]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S2340-98942019000200079</article-id>
<article-id pub-id-type="doi">10.30827/ars.v60i2.7920</article-id>
<title-group>
<article-title xml:lang="en"><![CDATA[The determination of benzalkonium chloride in eye drops]]></article-title>
<article-title xml:lang="es"><![CDATA[Determinación del cloruro de benzalconio en los colirios]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Mykola]]></surname>
<given-names><![CDATA[Blazheyevskiy]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Olena]]></surname>
<given-names><![CDATA[Koval&#8217;ska]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,National University of Pharmacy Department of Physical and Colloid Chemistry ]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
<country>Ucrania</country>
</aff>
<aff id="Af2">
<institution><![CDATA[,National University of Pharmacy Department of Inorganic Chemistry ]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
<country>Ucrania</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>06</month>
<year>2019</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>06</month>
<year>2019</year>
</pub-date>
<volume>60</volume>
<numero>2</numero>
<fpage>79</fpage>
<lpage>84</lpage>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://scielo.isciii.es/scielo.php?script=sci_arttext&amp;pid=S2340-98942019000200079&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://scielo.isciii.es/scielo.php?script=sci_abstract&amp;pid=S2340-98942019000200079&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://scielo.isciii.es/scielo.php?script=sci_pdf&amp;pid=S2340-98942019000200079&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="en"><p><![CDATA[ABSTRACT  Aim: A novel sensitive kinetic photometric method for the benzalkonium chloride determination has been developed.  Materials and method: The method is based on the ability to inhibit the reaction of acetylcholine hydrolysis by cholinesterase. The reaction rate is evaluated by the non-hydrolysed acetylcholine residue, which is determined by the amount of Peracetic acid, produced during the interaction with the excess of solution hydrogen peroxide . Indicator reaction is an interaction of p-phenetidine with Peracetic acid that leads to the formation of 4,4&#8217;-azoxyphenetole with &#955;max = 358 nm (log10 &#949; =4.2).  Results:  The conditions affecting the reaction (reagents concentration, pH, order of addition of reagents, stability in time) have been optimized. The linear dependence has been obeyed in the range of (1.4 to 8.4)·10-6 of benzalkonium chloride with correlation coefficient of 0.999. The assay limit of quantitation (20 % of the inhibition degree) has been 1.9·10-6 mol L-1.  Conclusion:  The proposed method has been successfully applied to the analysis for eye drops and allowed to confirm accuracy and reliability of the results obtained.]]></p></abstract>
<abstract abstract-type="short" xml:lang="es"><p><![CDATA[RESUMEN  Objetivos:  Se ha desarrollado un nuevo método fotométrico cinético sensible para la determinación de la presencia del cloruro de benzalconio.  Material y métodos:  El método se basa en la capacidad de inhibir la hidrolisis de acetilcolina en presencia de colinesterasa. La velocidad de reacción se estima mediante el residuo no hidrolizado de acetilcolina, que está determinado por la cantidad de ácido peracetico formado durante la interacción de la acetilcolina con el exceso de peróxido de hidrogeno. La reacción indicativa es la interacción de -etoxianilina con ácido peracetico, que da como resultado la formación de 4,4&#8217;-azoxifenetol con &#955;max = 358 nm ((log10 =4.2).  Resultados:  Las condiciones óptimas seleccionadas para llevar a cabo el experimento. El experimento se llevó a cabo en el rango de concentraciones (1.4 to 8.4·10-6 mol L-1 de cloruro de benzalconio. el coeficiente de correlación fue 0.999. Los límites de la definición de análisis (20% del grado de inhibición) fueron 1.9·10-6 mol L-1.  Conclusiones:  El método propuesto se aplicó con éxito en el análisis de gotas oculares &#1091; permitió confirmar la precisión &#1091; fiabilidad de los resultados.]]></p></abstract>
<kwd-group>
<kwd lng="en"><![CDATA[Benzalkonium chloride]]></kwd>
<kwd lng="en"><![CDATA[cholinesterase]]></kwd>
<kwd lng="en"><![CDATA[acetylcholine]]></kwd>
<kwd lng="es"><![CDATA[Benzalkonium chloride]]></kwd>
<kwd lng="es"><![CDATA[cholinesterase]]></kwd>
<kwd lng="es"><![CDATA[acetylcholine]]></kwd>
</kwd-group>
</article-meta>
</front><back>
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