<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>2340-9894</journal-id>
<journal-title><![CDATA[Ars Pharmaceutica (Internet)]]></journal-title>
<abbrev-journal-title><![CDATA[Ars Pharm]]></abbrev-journal-title>
<issn>2340-9894</issn>
<publisher>
<publisher-name><![CDATA[Universidad de Granada]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S2340-98942021000300260</article-id>
<article-id pub-id-type="doi">10.30827/ars.v62i3.16866</article-id>
<title-group>
<article-title xml:lang="en"><![CDATA[Application enzymatic method for analysis vaginal tabletas "fluomizin"]]></article-title>
<article-title xml:lang="es"><![CDATA[Aplicación método enzimático para análisis comprimidos vaginales "fluomizin"]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Blazheyevsk&#1110;y]]></surname>
<given-names><![CDATA[Mykola]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Koval'ska]]></surname>
<given-names><![CDATA[Olen&#1072;]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,National University of Pharmacy Phamaceutical Faculty Department of Physical and Colloid Chemistry]]></institution>
<addr-line><![CDATA[Kharkiv ]]></addr-line>
<country>Ukraine</country>
</aff>
<aff id="Af2">
<institution><![CDATA[,National University of Pharmacy Phamaceutical Faculty Department of Inorganic Chemistry]]></institution>
<addr-line><![CDATA[Kharkiv ]]></addr-line>
<country>Ukraine</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>09</month>
<year>2021</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>09</month>
<year>2021</year>
</pub-date>
<volume>62</volume>
<numero>3</numero>
<fpage>260</fpage>
<lpage>269</lpage>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://scielo.isciii.es/scielo.php?script=sci_arttext&amp;pid=S2340-98942021000300260&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://scielo.isciii.es/scielo.php?script=sci_abstract&amp;pid=S2340-98942021000300260&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://scielo.isciii.es/scielo.php?script=sci_pdf&amp;pid=S2340-98942021000300260&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="en"><p><![CDATA[Abstract  Introducción:  Desarrollo y validación de un nuevo método cinético-fotométrico enzimático para la determinación cuantitativa de cloruro de decualinio en tabletas vaginales "Fluomizin", basado en la inhibición de la reacción de hidrólisis enzimática de acetilcolina.  Método:  El contenido de cloruro de dequalinio en las tabletas "Fluomizin" &#8470; 6 se determinó por el grado de inhibición de la reacción enzimática, que se evaluó por la cantidad residual de acetilcolina en la reacción bioquímica. Como indicador, se usó la reacción de oxidación de p-fenetidina con ácido peracético, que se forma durante la reacción auxiliar de perhidrólisis con exceso de peróxido de hidrógeno durante un cierto período de tiempo en la mezcla de reacción.  Resultados:  Se determinaron las condiciones óptimas para la determinación: pH seleccionado 8,35, concentración de acetilcolina (0,05 mg / ml), colinesterasa (0,4 mg / ml), peróxido de hidrógeno (10%) y p-fenetidina (1%)tiempo de reacción (20 min).  Conclusiones:  Un método enzimático cinético-fotométrico para determinar el contenido de cloruro de dequalinium en píldoras vaginales "Fluomizin" &#8470; 6. La validación del método desarrollado para parámetros tales como linealidad, precisión, precisión y límite de cuantificación. Se determinaron los parámetros cinéticos Km (constante de Michaelis-Menten) y Vmax (velocidad máxima).]]></p></abstract>
<abstract abstract-type="short" xml:lang="es"><p><![CDATA[Abstract  Introduction:  Development of a new enzymatic kinetic-photometric method for analysis of Dequalinium chloride, based on the use of enzymatic hydrolysis of acetylcholine, for determine the amount of Dequalinium chloride in the vaginal tablets "Fluomizin" No 6.  Method:  The amount of Dequalinium chloride (DCh) was determined by the degree of inhibition of the enzymatic reaction, which was evaluated by the residual unreacted substrate - acetylcholine. Determination of the residual amount of acetylcholine in the reaction mixture was performed by a kinetic-photometric method using an indicator oxidation reaction of p-phenetidine with peracetic acid, which is formed during the auxiliary reaction of perhydrolysis with addition of excess hydrogen peroxide in the reaction mixture over a period of time.  Results:  Optimal conditions were determined: pH 8.35 was selected, influence of nature of buffer solution, concentration of acetylcholine (0.05 mg / ml), cholinesterase (0.4 mg / ml), hydrogen peroxide (10,0%) and p-phenethedine (1 %), the incubation time (10 min).  Conclusions: The procedure for determination of Dequalinium chloride content in the vaginal tablets "Fluomizin" No 6 was developed. The kinetic parameters Km (Michaelis-Menten constant) and Vmax (maximal velocity) were determined.]]></p></abstract>
<kwd-group>
<kwd lng="en"><![CDATA[cloruro de dequalinio]]></kwd>
<kwd lng="en"><![CDATA[fluomizina]]></kwd>
<kwd lng="en"><![CDATA[método cinético-fotométrico enzimático]]></kwd>
<kwd lng="es"><![CDATA[Dequalinium chloride]]></kwd>
<kwd lng="es"><![CDATA[Fluomizin]]></kwd>
<kwd lng="es"><![CDATA[enzymatic-photometric method]]></kwd>
</kwd-group>
</article-meta>
</front><back>
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