<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>2340-9894</journal-id>
<journal-title><![CDATA[Ars Pharmaceutica (Internet)]]></journal-title>
<abbrev-journal-title><![CDATA[Ars Pharm]]></abbrev-journal-title>
<issn>2340-9894</issn>
<publisher>
<publisher-name><![CDATA[Universidad de Granada]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S2340-98942023000200005</article-id>
<article-id pub-id-type="doi">10.30827/ars.v64i2.27401</article-id>
<title-group>
<article-title xml:lang="en"><![CDATA[Antagonistic effect of mepyramine and tubocurarine on the histamine-mediated contraction of intestinal smooth muscle cells]]></article-title>
<article-title xml:lang="es"><![CDATA[Efecto antagónico de mepiramina y tubocurarina sobre la contracción del músculo liso intestinal mediado por histamina]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Salvatierra]]></surname>
<given-names><![CDATA[Andreé]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Universidad Autónoma de Barcelona  ]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
<country>España</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>06</month>
<year>2023</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>06</month>
<year>2023</year>
</pub-date>
<volume>64</volume>
<numero>2</numero>
<fpage>139</fpage>
<lpage>147</lpage>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://scielo.isciii.es/scielo.php?script=sci_arttext&amp;pid=S2340-98942023000200005&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://scielo.isciii.es/scielo.php?script=sci_abstract&amp;pid=S2340-98942023000200005&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://scielo.isciii.es/scielo.php?script=sci_pdf&amp;pid=S2340-98942023000200005&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="en"><p><![CDATA[Abstract  Introduction: Histamine is an endogenous ligand which exerts its biological actions by binding to 4 subtypes of histamine receptors (HR). Its effects on the gastrointestinal tract are complex being the dominant effect contraction of muscle cells.  Method: The aim of this study was to verify the antagonistic effect of mepyramine (10-10M - 10-7M) and tubocurarine (10-7M - 10-4M) in the contractile action of histamine (10-11M - 10-4M). in guinea pig ileum, for this purpose it was used the program Virtual Organ Bath 16 V 2.8, which allow quantify the change in strength(g) produced by smooth muscle contraction in the presence of these substances. Concentration-response curves were obtained through GraphPad Prism® graphic program. The determination of the competitive nature of the antagonist and the calculation of the affinity constant was performed through Schild method.  Results: Mepyramine is a competitive antagonist of histamine (10-10M to 10-7M), making it a potential therapeutic agent for the treatment of irritable bowel syndrome. On the other hand, it is not understood that tubocurarine is an antagonist of histamine receptors.  Conclusions: The results obtained provide evidence that the contractile effect of histamine on the smooth muscle of intestinal cells isolated from guinea pig is competitively antagonized by mepyramine. In addition, by calculating the pKB parameter, it was found that this compound has greater potency than other antihistamines.]]></p></abstract>
<abstract abstract-type="short" xml:lang="es"><p><![CDATA[Resumen  Introducción: La histamina es un ligando endógeno que ejerce sus acciones biológicas uniéndose a 4 subtipos de receptores de histamina (HR), sus efectos en el tracto gastrointestinal son complejos siendo el efecto dominante la contracción de las células musculares.  Método: El objetivo de este estudio fue verificar el efecto antagónico de la mepiramina (10-10M - 10-7M) y la tubocurarina (10-7M - 10-4M) en la acción contráctil de la histamina (10-11M - 10-4M) en el íleon de cobaya, para ello se utilizó el programa Virtual Organ Bath 16 V 2.8 que permitió cuantificar el cambio de fuerza (g) producido por la contracción del músculo liso en presencia de estas sustancias. Las curvas de concentración-respuesta se obtuvieron mediante el uso del programa gráfico GraphPad Prism®. La determinación del carácter competitivo del antagonista y el cálculo de la constante de afinidad se realizaron mediante el método de Schild.  Resultados: La mepiramina es un antagonista competitivo de la histamina (10-10M a 10-7M), convirtiéndola en un agente terapéutico potencial para el tratamiento del síndrome de intestino irritable. Por otro lado, no se encuentra que la tubocurarina sea un antagonista de los receptores de histamina.  Conclusiones: Los resultados obtenidos proporcionan evidencia acerca de que el efecto contráctil de la histamina, sobre el músculo liso de células intestinales aisladas en cobaya, es antagonizado competitivamente por la mepiramina. Además, mediante el cálculo del parámetro pKB se encontró que este compuesto presenta mayor potencia que otros antihistamínicos.]]></p></abstract>
<kwd-group>
<kwd lng="en"><![CDATA[Mepyramine]]></kwd>
<kwd lng="en"><![CDATA[tubocurarine]]></kwd>
<kwd lng="en"><![CDATA[histamine]]></kwd>
<kwd lng="en"><![CDATA[irritable bowel syndrome]]></kwd>
<kwd lng="es"><![CDATA[Mepiramina]]></kwd>
<kwd lng="es"><![CDATA[tubocurarina]]></kwd>
<kwd lng="es"><![CDATA[histamina]]></kwd>
<kwd lng="es"><![CDATA[síndrome del intestino irritable]]></kwd>
</kwd-group>
</article-meta>
</front><back>
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