<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>2529-850X</journal-id>
<journal-title><![CDATA[Journal of Negative and No Positive Results]]></journal-title>
<abbrev-journal-title><![CDATA[JONNPR]]></abbrev-journal-title>
<issn>2529-850X</issn>
<publisher>
<publisher-name><![CDATA[Research and Science S.L.]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S2529-850X2021000700003</article-id>
<article-id pub-id-type="doi">10.19230/jonnpr.3860</article-id>
<title-group>
<article-title xml:lang="es"><![CDATA[Cuantificación de la actividad específica de lectina en teosinte Zea diploperennis]]></article-title>
<article-title xml:lang="en"><![CDATA[Quantification of specific lectin activity in teosinte Zea diploperennis]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Matías-Luis]]></surname>
<given-names><![CDATA[Gabriel]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Pérez-Santiago]]></surname>
<given-names><![CDATA[Alma Dolores]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Pina-Canseco]]></surname>
<given-names><![CDATA[María del Socorro]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Castro-Juárez]]></surname>
<given-names><![CDATA[Carlos Jonnathan]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Sánchez-Medina]]></surname>
<given-names><![CDATA[Marco Antonio]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[García-Montalvo]]></surname>
<given-names><![CDATA[Iván Antonio]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Pérez-Campos]]></surname>
<given-names><![CDATA[Eduardo]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Tecnológico Nacional de México Instituto Tecnológico de Oaxaca Unidad de Bioquímica e Inmunología]]></institution>
<addr-line><![CDATA[Oaxaca de Juárez Oaxaca]]></addr-line>
<country>México</country>
</aff>
<aff id="Af2">
<institution><![CDATA[,Universidad Autónoma &#8220;Benito Juárez&#8221; de Oaxaca Facultad de Medicina y Cirugía Centro de Investigación Facultad de Medicina UNAM-UABJO]]></institution>
<addr-line><![CDATA[Oaxaca de Juárez Oaxaca]]></addr-line>
<country>México</country>
</aff>
<aff id="Af3">
<institution><![CDATA[,Universidad de la Sierra Sur Instituto de Nutrición ]]></institution>
<addr-line><![CDATA[Miahuatlán de Porfirio Díaz Oaxaca]]></addr-line>
<country>México</country>
</aff>
<aff id="Af4">
<institution><![CDATA[,Instituto Tecnológico de Oaxaca Departamento de Ingeniería Química y Bioquímica Laboratorio de Alimentos]]></institution>
<addr-line><![CDATA[Oaxaca de Juárez Oaxaca]]></addr-line>
<country>México</country>
</aff>
<aff id="Af5">
<institution><![CDATA[,Laboratorio de Patología Clínica &#8220;Dr. Eduardo Pérez Ortega&#8221;  ]]></institution>
<addr-line><![CDATA[Oaxaca de Juárez Oaxaca]]></addr-line>
<country>México</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>00</month>
<year>2021</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>00</month>
<year>2021</year>
</pub-date>
<volume>6</volume>
<numero>7</numero>
<fpage>941</fpage>
<lpage>950</lpage>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://scielo.isciii.es/scielo.php?script=sci_arttext&amp;pid=S2529-850X2021000700003&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://scielo.isciii.es/scielo.php?script=sci_abstract&amp;pid=S2529-850X2021000700003&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://scielo.isciii.es/scielo.php?script=sci_pdf&amp;pid=S2529-850X2021000700003&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="es"><p><![CDATA[Resumen  Objetivo Evaluar la actividad específica de lectina en teosinte Zea diploperennis sano e infectado con Ustilago maydis.  Materiales y métodos. Plántulas de Zea diploperennis de 6 días de crecimiento fueron inoculadas con Ustilago maydis. Se evaluó la concentración de proteínas totales y la actividad hemaglutinante de extractos crudos de teosinte sano e infectado en placas de microtitulación con eritrocitos tipo O al 3% siguiendo la técnica de diluciones dobles seriadas durante 6 días.  Resultados. La concentración de proteínas totales se incrementa en coleoptilo sano durante cada día de su crecimiento. No así en teosinte infectado donde la curva presenta una tendencia a la baja desde el momento de la inoculación. La actividad específica de lectina disminuye en ambos casos desde el primer día de cuantificación.  Conclusiones. La evidente reducción en la actividad de lectina en teosinte infectado en comparación con teosinte sano podría explicar la susceptibilidad de este teosinte a dicho fitopatógeno. Si bien, la participación de las lectinas de teosinte y maíz en el mecanismo de defensa a Ustilago maydis todavía no ha sido esclarecida, los resultados obtenidos contribuyen a la comprensión del efecto que puede tener la concentración de lectina y proteína sobre la resistencia en teosinte.]]></p></abstract>
<abstract abstract-type="short" xml:lang="en"><p><![CDATA[Abstract  Objective. To evaluate the specific activity of lectin in healthy and infected teosinte Zea diploperennis with Ustilago maydis.  Materials and methods. Zea diploperennis seedlings of 6 days of growth were inoculated with Ustilago maydis. Total protein concentration and hemagglutinating activity of crude extracts of healthy and infected teosinte were evaluated in microtiter plates with 3% type O red cells following the technique of serial double dilutions for 6 days.  Results. The concentration of total proteins increases in healthy coleoptile during each day of its growth. Not so in infected teosinte where the curve shows a downward trend from the moment of inoculation. The specific lectin activity decreases in both cases from the first day of quantification.  Conclusions. The evident reduction in lectin activity in infected teosinte compared to healthy teosinte could explain the susceptibility of this teosinte to said phytopathogen. Although the participation of the teosinte and corn lectins in the defense mechanism against Ustilago maydis has not yet been clarified, the results obtained contribute to the understanding of the effect that the concentration of lectin and protein can have on resistance in teosinte.]]></p></abstract>
<kwd-group>
<kwd lng="es"><![CDATA[Actividad de lectina]]></kwd>
<kwd lng="es"><![CDATA[proteína total]]></kwd>
<kwd lng="es"><![CDATA[Zea diploperennis]]></kwd>
<kwd lng="es"><![CDATA[Ustilago maydis]]></kwd>
<kwd lng="en"><![CDATA[Lectin activity]]></kwd>
<kwd lng="en"><![CDATA[total protein]]></kwd>
<kwd lng="en"><![CDATA[Zea diploperennis]]></kwd>
<kwd lng="en"><![CDATA[Ustilago maydis]]></kwd>
</kwd-group>
</article-meta>
</front><back>
<ref-list>
<ref id="B1">
<label>1</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Goldstein]]></surname>
<given-names><![CDATA[I. J]]></given-names>
</name>
<name>
<surname><![CDATA[Hughes]]></surname>
<given-names><![CDATA[R. C]]></given-names>
</name>
<name>
<surname><![CDATA[Monsiony]]></surname>
<given-names><![CDATA[M]]></given-names>
</name>
<name>
<surname><![CDATA[Osawa]]></surname>
<given-names><![CDATA[T]]></given-names>
</name>
<name>
<surname><![CDATA[Sharon]]></surname>
<given-names><![CDATA[N]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[What should be called a lectin?]]></article-title>
<source><![CDATA[Nature]]></source>
<year>1980</year>
<volume>285</volume>
</nlm-citation>
</ref>
<ref id="B2">
<label>2</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Chrispeels]]></surname>
<given-names><![CDATA[M. J]]></given-names>
</name>
<name>
<surname><![CDATA[Raihhel]]></surname>
<given-names><![CDATA[N. V]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Lectins, lectin genes, and their role in plant defense]]></article-title>
<source><![CDATA[Plant Cell]]></source>
<year>1991</year>
<volume>3</volume>
<numero>1</numero>
<issue>1</issue>
<page-range>1-9</page-range></nlm-citation>
</ref>
<ref id="B3">
<label>3</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Pérez-Santiago]]></surname>
<given-names><![CDATA[A]]></given-names>
</name>
<name>
<surname><![CDATA[Saavedra]]></surname>
<given-names><![CDATA[E]]></given-names>
</name>
<name>
<surname><![CDATA[Pérez-Campos]]></surname>
<given-names><![CDATA[E]]></given-names>
</name>
<name>
<surname><![CDATA[Córdoba]]></surname>
<given-names><![CDATA[F]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Effect of plant lectins on Ustilago maydis in Vitro]]></article-title>
<source><![CDATA[Cell Mol. Life Sci]]></source>
<year>2000</year>
<volume>57</volume>
<page-range>1986-99</page-range></nlm-citation>
</ref>
<ref id="B4">
<label>4</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Martínez-Cruz]]></surname>
<given-names><![CDATA[M]]></given-names>
</name>
<name>
<surname><![CDATA[Zenteno]]></surname>
<given-names><![CDATA[E]]></given-names>
</name>
<name>
<surname><![CDATA[Córdoba]]></surname>
<given-names><![CDATA[F]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Purification and characterization of a galactose-specific lectin from corn (Zea mays) coleoptile]]></article-title>
<source><![CDATA[Biochim. Byophys. Acta]]></source>
<year>2001</year>
<volume>1568</volume>
<page-range>37-44</page-range></nlm-citation>
</ref>
<ref id="B5">
<label>5</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Martinez-Cruz]]></surname>
<given-names><![CDATA[M]]></given-names>
</name>
<name>
<surname><![CDATA[Cordoba]]></surname>
<given-names><![CDATA[F]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Isolation of fully active and stable teosinte coleoptile lectins]]></article-title>
<source><![CDATA[Prep. Biochem. Biotechnol]]></source>
<year>2000</year>
<volume>30</volume>
<page-range>199-208</page-range></nlm-citation>
</ref>
<ref id="B6">
<label>6</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Iltis]]></surname>
<given-names><![CDATA[H. H]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Homeotic sexual translocations and the origin of maize (Zea mays, Poaceae): A new look at an old problem]]></article-title>
<source><![CDATA[Econ. Bot]]></source>
<year>2000</year>
<volume>54</volume>
<page-range>7-42</page-range></nlm-citation>
</ref>
<ref id="B7">
<label>7</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Pérez Díaz]]></surname>
<given-names><![CDATA[MC]]></given-names>
</name>
<name>
<surname><![CDATA[Sánchez Medina]]></surname>
<given-names><![CDATA[MA]]></given-names>
</name>
<name>
<surname><![CDATA[Pina Canseco]]></surname>
<given-names><![CDATA[MS]]></given-names>
</name>
<name>
<surname><![CDATA[Pérez Campos]]></surname>
<given-names><![CDATA[E]]></given-names>
</name>
<name>
<surname><![CDATA[García Montalvo]]></surname>
<given-names><![CDATA[IA]]></given-names>
</name>
<name>
<surname><![CDATA[Pérez Santiago]]></surname>
<given-names><![CDATA[AD]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Inducción de infección en Teosinte (Zea diploperennis) con el fitopatógeno Ustilago maydis]]></article-title>
<source><![CDATA[JONNPR]]></source>
<year>2017</year>
<volume>1</volume>
<numero>1</numero>
<issue>1</issue>
<page-range>17-22</page-range></nlm-citation>
</ref>
<ref id="B8">
<label>8</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Iltis]]></surname>
<given-names><![CDATA[H. H]]></given-names>
</name>
<name>
<surname><![CDATA[Doebley]]></surname>
<given-names><![CDATA[J. F]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Taxonomic of Zea (Gramineae) II. Subspecifics categories in the Zea mays complex and a generic synopsis]]></article-title>
<source><![CDATA[Amer. J. Bot]]></source>
<year>1980</year>
<volume>67</volume>
<page-range>994-1004</page-range></nlm-citation>
</ref>
<ref id="B9">
<label>9</label><nlm-citation citation-type="book">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Matías]]></surname>
<given-names><![CDATA[L. G]]></given-names>
</name>
</person-group>
<source><![CDATA[Inducción de la infección en coleoptilos de teosinte Zea diploperennis con Ustilago maydis]]></source>
<year>2013</year>
<publisher-name><![CDATA[Instituto Tecnológico de Oaxaca]]></publisher-name>
</nlm-citation>
</ref>
<ref id="B10">
<label>10</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Bradford]]></surname>
<given-names><![CDATA[M. M]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding]]></article-title>
<source><![CDATA[Anal Biochem]]></source>
<year>1976</year>
<volume>72</volume>
<page-range>248-54</page-range></nlm-citation>
</ref>
<ref id="B11">
<label>11</label><nlm-citation citation-type="confpro">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Cruz]]></surname>
<given-names><![CDATA[M. M. C]]></given-names>
</name>
<name>
<surname><![CDATA[Pérez]]></surname>
<given-names><![CDATA[D. M. C]]></given-names>
</name>
<name>
<surname><![CDATA[Matías]]></surname>
<given-names><![CDATA[L. G]]></given-names>
</name>
<name>
<surname><![CDATA[Santiago]]></surname>
<given-names><![CDATA[O. G]]></given-names>
</name>
<name>
<surname><![CDATA[Aragón]]></surname>
<given-names><![CDATA[C. F]]></given-names>
</name>
<name>
<surname><![CDATA[Sánchez]]></surname>
<given-names><![CDATA[M. M. A]]></given-names>
</name>
<name>
<surname><![CDATA[Pina]]></surname>
<given-names><![CDATA[C. S]]></given-names>
</name>
<name>
<surname><![CDATA[Pérez]]></surname>
<given-names><![CDATA[S. A. D]]></given-names>
</name>
</person-group>
<source><![CDATA[Gene expression per1 in teocintle (Zea mays ssp parviglumis) infected with Ustilago maydis]]></source>
<year>2013</year>
<conf-name><![CDATA[ XVI Congreso Internacional y XLI Congreso Nacional; Sociedad Mexicana de Fitopatología]]></conf-name>
<conf-loc> </conf-loc>
<publisher-name><![CDATA[Unidad de Bioquímica e Inmunología, Instituto Tecnológico de Oaxaca]]></publisher-name>
</nlm-citation>
</ref>
</ref-list>
</back>
</article>
