Hepatic fatty acid profile of rats fed a triheptanoin-based ketogenic diet
Perfil de ácidos grasos de hígados de ratones alimentados con una dieta cetogénica basada en triheptanoína
Ingrid Sofia Vieira de Meloi1, Terezinha da Rocha Ataidei2, Suzana Lima de Oliveirai2, Nassib Bezerra Buenoi2, Johnnatan Duarte de Freitasi3 and Antônio Euzébio Goulart Sant'Anai4
1Departamento de Agroindústria, Instituto Federal de Educação Ciência e Tecnologia de Alagoas, Murici, AL.
2Laboratório de Nutrição Experimental, Faculdade de Nutrição, Universidade Federal de Alagoas, Maceió, AL.
3Departamento de Tecnologia de Alimentos, Instituto Federal de Educação Ciência e Tecnologia de Alagoas, Maceió - AL. ]]>
4Laboratório de Recursos Naturais, Instituto de Química e Biotecnologia, Universidade Federal de Alagoas, Maceió, AL, Brasil.
ABSTRACT
Objective: the aim of this study was to evaluate the influence of consumption of a ketogenic diet supplemented with triheptanoin, a medium-chain anaplerotic triacylglycerol, on the liver fatty acid profile of Wistar rats.
Methods: three groups of male Wistar rats (n = 10) were submitted to an AIN-93 control diet, a triheptanoin- based ketogenic diet, or a soybean oil-based ketogenic diet for 60 days. Excised livers were subjected to lipid extraction and methylation to obtain fatty acids methyl esters, which were subjected to gas chromatography- mass spectrometry.
Results and discussion: compared to the rats fed the control diet, those fed ketogenic diets showed a significant reduction in the concentrations of 9-hexadecenoic and 9-octadecenoic acids, whereas those fed triheptanoin showed increased levels of octadecanoic acid.
Conclusions: changes in the liver fatty acid profiles of the rats fed a triheptanoin-based or a soybean oil-based ketogenic diet did not seem to be related to the dietary fat source, but rather to the characteristics of the ketogenic diets themselves.
Key words: Fatty acid profile. Ketogenic diet. Medium-chain triacylglycerols. Trienantin.
RESUMEN
]]> Objetivo: el objetivo de este estudio fue evaluar la influencia del consumo de una dieta cetogénica complementada con triheptanoína, un triacilglicerol de cadena media y anaplerótico, en el perfil de ácidos grasos del hígado de ratones Wistar.Palabras clave: Ratones Wistar. Triheptanoína. Ácidos grasos.
http://scielo.isciii.es/pdf/nh/v32n1/38originalinvestigacionanimal02.pdf
]]>