Abstract

YEHIA, Mona Abdel-Hamed; AL-QADASI, Sabah Ali  and  AL-SEDFY, Amel Sobhey. Quantitative immunohistochemical expression of GP88 in invasive ductal carcinoma (IDC) of human breast cancer. Iberoam J Med [online]. 2021, vol.3, n.1, pp.33-43.  Epub Dec 25, 2023. ISSN 2695-5075.  https://dx.doi.org/10.5281/zenodo.4422074.

Introduction: Progranulin or acrogranin is an 88-kDa glycoprotein identified by a biological screen for protein targets associated with high tumorigenicity. This work was aimed to investigate the digital expression of GP88, and HER2/neu as a predictive biomarker in human invasive ductal carcinoma (IDC) versus benign tumors and normal breast tissues, as well as its correlation with different pathological parameters.

Methods: The immunohistochemical avidin-biotin complex protocol of the paraffin section was used to detect the expression of GP88 and HER2/neu in IDC of 60 patients, 30 benign, and ten normal breast tissues.

Results: The study showed a high expression of GP88 in IDC comparing to normal and benign breast tissues. A higher significant statistical correlation between the expression of GP88 and large tumor size, tumor grade, and lymph node metastasis (LNM). While a negative statistical correlation was noticed between the expression of GP88 and ER, PR, and HER2/neu status.

Discussion: The results of the quantitative immunostaining density of GP88 glycoprotein antibody revealed it to be a valuable predictive and therapeutic marker of GP88 in human IDC patients.

Keywords : HER2/neu; GP88; Clinical pathological parameter; Invasive ductal carcinoma; Immunohistochemical staining; Integrated optical density.

        · text in English     · English ( pdf )